Microscope ya fluorescence ni ubwoko bwa microscope optique ikoresha isoko yumucyo mwinshi kugirango imurikire urugero kandi ishimishe fluorochromes murugero. Kumurika by'icyitegererezo mubisanzwe bikorwa hamwe numucyo utanga urumuri ultraviolet. Zikoreshwa cyane mubinyabuzima, ubuvuzi ninganda.
Nigute microscope ya fluorescence ikora?
Microscope ya fluorescence ikoresha itara rya mercure cyangwa xenon kugirango itange urumuri ultraviolet. Umucyo uza muri microscope ugakubita indorerwamo ya dicroic - indorerwamo yerekana urwego rumwe rw'uburebure kandi ikemerera urundi rugendo kunyuramo. Indorerwamo ya dichroic yerekana urumuri ultraviolet kugeza kurugero. Bimwe mubigereranyo fluoresce mubisanzwe munsi yumucyo ultraviolet kuko irimo ibintu bya fluorescent nka chlorophyll. Niba urugero rugomba kurebwa rudasanzwe rwa fluoresce, rushobora kwanduzwa irangi rya fluorescent bita fluorochromes.
Microscope ya fluorescence igizwe niki?
1. Inkomoko yumucyo
Inkomoko yumucyo ikunze kugaragara ni mercure, xenon, na LED. Mercure itanga urumuri rwiza rwa microscope ya fluorescence. LED iragenda ikundwa cyane kuko ihendutse kuruta andi masoko kandi ikoresha ingufu nke.
| Itara rya Mercure | Xenon Itara | LED | |
| Uburebure bwumucyo wasohotse | 350-370 nm | 400nm ～ 450nm | 400-700 nm |
| Ubuzima bwose (isaha) | 200-300 | 400-600 | 10000 |
| Ibyiza | 1. Umucyo mwinshi cyane, urumuri rusanzwe ruturuka. 2. Isohora ultraviolet ikomeye nubururu-violet kugirango ishimishe ubwoko bwose bwa fluorofore. 3. Amashusho meza kandi afite amabara. | 1. Ubwinshi bwikigereranyo burahagaze. 2. Imbaraga zikomeye zerekana muri infragre na mid-infragre. | 1. Biroroshye gusimbuza. 2. Ntabwo ari ngombwa gushyuha. 3. Irashobora gufungura no kuzimya ako kanya. 4. Kugenzura ubukana bwumuriro. |
| Ingaruka | 1. Ubuzima Bugufi. 2. Kumara igihe kinini. | 1. Agasanduku kadasanzwe k'amashanyarazi DC gasabwa kugirango ubushyuhe burenze. 2. Birahenze kuruta Itara rya Mercure | 1. Itara rya UV rifite intege nke kuruta itara rya mercure. 2.Buri burebure bwumucyo bisaba LED itandukanye bitewe numuyoboro mugari basohora. |
Umugereka wa Fluorescent Umugereka
LED Umugereka wa Fluorescent
2. Akayunguruzo gashimishije
Akayunguruzo gashimishije ni ngombwa mu mikorere ya microscope ya fluorescence. Itambutsa urumuri rw'uburebure bugufi, irangi rya fluorescent rishobora gukuramo. Kandi, irabuza andi masoko yumucyo ushimishije.
Akayunguruzo gashimishije (B, G, U, V)
3. Indorerwamo ya Dichroic
Indorerwamo ya dichroic ni ubwoko bwa optique ya filteri yerekana urumuri kumurambararo runaka mugihe wohereza izindi. Ikoreshwa muri microscopes ya fluorescence kugirango itandukane uburebure bwimyuka n’ibyuka.
4. Akayunguruzo
Akayunguruzo gasohoka kanyura gusa uburebure bwumurongo utangwa na fluorophore kandi bugahagarika urumuri rutifuzwa hanze yiri tsinda - cyane cyane uburebure bwumuriro.
5. Amabara ya Fluorescent
Irangi rya Fluorescent ni ibinyabuzima bifite imiterere ya fluorescence, aho ishobora gukora ishusho ya fluorescente itanga urumuri rutandukanye cyane rwicyatsi kibisi nyuma yo gushimishwa nurumuri ultraviolet rumurika cyane. Amabara akoreshwa cyane ya fluorescent ni; DAPI (49,6-diamidino-2-fenylindole), acridine orange, auramine-rhodamine, Alexa Fluors, cyangwa DyLight 488.
Ubwoko bwa microscopes bwa fluorescence bangahe?
1. Microscope Yuzuye Epifluorescence:
Nubwoko busanzwe bwa microscope ya fluorescence. Ibyishimo bya fluorophore no kumenya fluorescence bikorwa binyuze mumuhanda umwe (nukuvuga intego). Ubwinshi bwa microscopes ya fluorescence, cyane cyane ikoreshwa mubumenyi bwubuzima, ni igishushanyo cya epifluorescence.
BS-2081F Ubushakashatsi Fluorescent Biologiya Microscope
2. Microscope ya Confocal Fluorescence:
Microscope ya Confocal Fluorescence: Ubu bwoko bwa microscope ya fluorescence ihuza laser yogusikana hamwe na florescent yamurika kugirango bitange ishusho. Irashobora gukoreshwa muburyo butandukanye bwa porogaramu, nko kwiga selile na tissue, kumenya poroteyine nibindi bintu biri muri selile, no gupima ubunini bwibikoresho.
BS-2081F Ubushakashatsi Fluorescent Biologiya Microscope
3. Microscope ihindagurika
Ubu bwoko bwa microscope yumucyo hamwe na kondenseri biri hejuru, bireba hepfo. Inguni yo kumurika igomba kuba kuri dogere 90 kubijyanye nubuso bwikigereranyo gisuzumwa.
BS-2095F Microscope ihindagurika
Ni izihe nyungu n'ibibi bya microscopes ya fluorescence?
Ibyiza:
- Gushoboza kwitegereza ingirabuzimafatizo mu binyabuzima bizima bitangiritse
- Itanga amashusho yikirenga afite amabara yukuri
- Emerera kwiga inzira nzima muri selile
- Koresha kugirango umenye ubwoko butandukanye bwa molekile imbere muri selile, nka proteyine cyangwa acide nucleic.
Ibibi:
-Byemerera gusa kureba imiterere yihariye imbere muri selile yashushanyijeho irangi rya fluorescent.
-Gufotora bitewe n'ibyishimo bya electron mugihe cya fluorescence birashobora kugira ingaruka kuri molekile zifatika zamabara ya fluorescent. Nkigisubizo, amarangi adasanzwe arashobora gutakaza imiti yimiti ya fluorescence yangiza.
-Ingirabuzimafatizo zishobora kwibasirwa n'ingaruka zifotora nyuma yo gusiga irangi rya fluorescent, kuko molekile ya fluorophore ikurura fotone yingufu nyinshi ziva mumucyo mugufi.
Ni ubuhe buryo bukoreshwa na microscope ya fluorescence?
Fluorescence microscopes ikoreshwa cyane mubice bitandukanye byubushakashatsi no kuyishyira mu bikorwa harimo ibinyabuzima, ibinyabuzima, selile, microbiologiya, immunologiya, n’ubuvuzi.
1. Mu rwego rwa biyolojiya, microscope ya fluorescence ituma umuntu amenya neza kandi arambuye ibice bigize selile na submicroscopic selile selile hamwe nibikorwa hifashishijwe irangi rya fluorescent.
2. Mu rwego rwubuvuzi, microscope ya fluorescence irashobora gukoresha reagent ya fluorescent kugirango hamenyekane ahari bagiteri na virusi, cyangwa gufasha mukwandika intego zo kubaga kugirango byoroherezwe kubagwa.
3. Mu rwego rwa mineralogi, microscope ya fluorescence ikoreshwa kenshi mukwiga ibintu bifite imiterere ya fluorescence itunguranye, nka asfalt, peteroli, amakara, okiside ya graphene nandi mabuye y'agaciro.
4. Mubikoresho siyanse, microscope ya fluorescence irashobora gukoreshwa mubikorwa byimyenda cyangwa inganda zimpapuro kugirango zisesengure ibikoresho bishingiye kuri fibre.
Igihe cyo kohereza: Werurwe-14-2023