Fluorescence microscopi yahinduye ubushobozi bwacu bwo kwiyumvisha no kwiga ingero z'ibinyabuzima, bidufasha gucengera mu isi igoye ya selile na molekile. Ikintu cyingenzi kigizwe na microscopi ya fluorescence nisoko yumucyo ikoreshwa mugushimisha molekile ya fluorescent murugero. Mu myaka yashize, hakoreshwa amasoko atandukanye yumucyo, buriwese ufite imiterere yihariye nibyiza.
1. Itara rya Mercure
Itara ryumuvuduko mwinshi wa mercure, kuva kuri watt 50 kugeza 200, ryubatswe hifashishijwe ikirahuri cya quartz kandi gifite imiterere. Harimo urugero runaka rwa mercure imbere. Iyo ikora, isohoka riba hagati ya electrode ebyiri, bigatuma mercure ihinduka, kandi umuvuduko wimbere murwego rwiyongera vuba. Iyi nzira mubisanzwe ifata iminota 5 kugeza kuri 15.
Isohora ry’itara ryinshi rya mercure rituruka ku gusenyuka no kugabanuka kwa molekile ya mercure mugihe cyo gusohora electrode, bigatuma habaho fotone yoroheje.
Isohora ultraviolet ikomeye nubururu-violet, bigatuma ikwiranye nibikoresho bitandukanye bya fluorescent, niyo mpamvu ikoreshwa cyane muri microscopi ya fluorescence.
2. Amatara ya Xenon
Iyindi mikoreshereze yumucyo wera muri microscopi ya fluorescence ni itara rya xenon. Amatara ya Xenon, kimwe n'amatara ya mercure, atanga umurongo mugari w'uburebure kuva kuri ultraviolet kugera hafi-ya-infragre. Ariko, baratandukanye mubyishimo byabo.
Amatara ya mercure yibanda cyane mu kirere hafi ya ultraviolet, ubururu, n'icyatsi kibisi, ibyo bikaba byerekana ibimenyetso bya fluorescent ariko bizana ifoto ikomeye. Kubera iyo mpamvu, amatara ya HBO asanzwe agenewe ingero zifatika cyangwa amashusho ya fluorescence idakomeye. Ibinyuranyo, itara rya xenon rifite umwirondoro ushimishije, ryemerera kugereranya ubukana kuburebure butandukanye. Ibiranga nibyiza kubikorwa nka calcium ion yibipimo byo gupima. Amatara ya Xenon yerekana kandi umunezero mwinshi murwego rwo hafi ya infragre, cyane cyane nm 800-1000 nm.
Amatara ya XBO afite ibyiza bikurikira kurenza amatara ya HBO:
① Imbaraga nyinshi zidasanzwe
Intens Gukomera kwinshi mu turere twa infragre na mid-infragre
Ingufu nyinshi zisohoka, byoroha kugera ku ntego ya aperture.
3. LED
Mu myaka yashize, hagaragaye umunywanyi mushya mubice bya microscopi microscopi ya fluorescence: LED. LEDs itanga ibyiza byo guhinduranya byihuse kuri milisegonda, kugabanya ibihe byo kwerekana no kongera igihe cyicyitegererezo cyiza. Byongeye kandi, urumuri rwa LED rugaragaza kubora vuba kandi neza, bigabanya cyane gufotora mugihe cyigihe kirekire cyakorewe selile.
Ugereranije n’umucyo wera, LED isanzwe isohora ibintu bigufi. Nyamara, imirongo myinshi ya LED iraboneka, itanga uburyo butandukanye bwo gukoresha amabara menshi ya fluorescence, bigatuma LED ihitamo gukundwa cyane muri microscopi ya kijyambere ya fluorescence.
4. Inkomoko yumucyo
Inkomoko yumucyo wa lazeri ni monochromatic cyane kandi ikayobora, bigatuma iba nziza kuri microscopi ihanitse cyane, harimo tekinoroji yo gukemura cyane nka STED (Stimulated Emission Depletion) na PALM (Photoactivated Localization Microscopy). Urumuri rwa Laser rusanzwe rwatoranijwe kugirango ruhuze umurambararo wihariye ukenewe kuri fluorophore, utanga amahitamo menshi kandi neza mubyishimo bya fluorescence.
Guhitamo florescence microscope yumucyo biterwa nibisabwa byihariye byubushakashatsi nibiranga icyitegererezo. Nyamuneka nyamuneka twandikire niba ukeneye ubufasha ubwo aribwo bwose
Igihe cyo kohereza: Nzeri-13-2023